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    lipofectamine stem

    This protocol uses the Lipofectamine Stem Transfection Reagent for efficient transfection of hiPSCs without the need for special instrumentation. It is appropriate for plasmid-based genome editing of hiPSCs cultured under xeno-free conditions on recombinant human laminin-521 in E8 medium, and can efficiently deliver the CRISPR-Cas9 plasmid (pX330) alone to induce insertions and deletions through NHEJ or with a pHDR construct to achieve HR-mediated modifications.

    • Hannah Cole
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    • Created 21 Sep 2023
    • Modified 21 Sep 2023
    • Hits 32